Effetct Of Two-Stroke Auyo Rikshaw Smoke Solution On Angiogenesis And Embryonic Movements Using Chicken
By: Khaleeq Anwar
| Prof.Dr.Muhammad Ashraf.
Contributor(s): Dr.Sheryar Afzal | Prof.Dr.Muhammad Sarwar.
Material type: 
BookPublisher: 2008Subject(s): Department of Pharmaoclogy & ToxicologyDDC classification: 1068,T Dissertation note: Auto rickshaw is a two-stroke vehicle widely used in South Asia for transportation. They emit 20-50% of their fuel unburned in the exhaust comprising a wide range of pollutants from simple to carcinogenic These pollutants of exhaust may have adverse effects on embryogenesis and angiogenesis. The current study was undertaken to assess the level of risk offered to pregnant women traveling and breathing in the environment condensed with emissions from auto rickshaws and to predict the possible teratological deformities and antiangiogenic effects followed by exposure to polluted air.
Smoke of auto rickshaw was taken in polythene bags containing distilled water and filtered. Filtered solution was used for experimental analysis. Fresh fertilized eggs were taken and incubated in incubator at 37C° and at 70-80% humidity. At day 4 of incubation 4-5 ml albumin was removed and a window was prepared. At day 6 of incubation, 200tl of samples was applied to the developing CAM, which was histological evaluated for any interim change in the process of angiogenesis. Similarly, for embryonic movements, eggs were incubated at 37C° and at 70-80% humidity. At day 5, 3-4m1 albumin was removed to visualize embryo. Egg was placed in incubator fitted with digital camera a video of embryonic movements was recorded for 1 hr. At 15 mm of recording, 200 ul of sample solution was applied on developing embryo and deviation in embryonic movements was recorded.
All above observation did undermine different toxicological activities of autorickshaw smoke solution on embryogenesis and angiogenesis.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1068,T (Browse shelf) | Available | 1068,T |
Auto rickshaw is a two-stroke vehicle widely used in South Asia for transportation. They emit 20-50% of their fuel unburned in the exhaust comprising a wide range of pollutants from simple to carcinogenic These pollutants of exhaust may have adverse effects on embryogenesis and angiogenesis. The current study was undertaken to assess the level of risk offered to pregnant women traveling and breathing in the environment condensed with emissions from auto rickshaws and to predict the possible teratological deformities and antiangiogenic effects followed by exposure to polluted air.
Smoke of auto rickshaw was taken in polythene bags containing distilled water and filtered. Filtered solution was used for experimental analysis. Fresh fertilized eggs were taken and incubated in incubator at 37C° and at 70-80% humidity. At day 4 of incubation 4-5 ml albumin was removed and a window was prepared. At day 6 of incubation, 200tl of samples was applied to the developing CAM, which was histological evaluated for any interim change in the process of angiogenesis. Similarly, for embryonic movements, eggs were incubated at 37C° and at 70-80% humidity. At day 5, 3-4m1 albumin was removed to visualize embryo. Egg was placed in incubator fitted with digital camera a video of embryonic movements was recorded for 1 hr. At 15 mm of recording, 200 ul of sample solution was applied on developing embryo and deviation in embryonic movements was recorded.
All above observation did undermine different toxicological activities of autorickshaw smoke solution on embryogenesis and angiogenesis.
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